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. 1999 Feb 1;103(3):429–435. doi: 10.1172/JCI2913

Figure 11.

Figure 11

Effect of media pH on apical membrane NHE3 protein abundance in the presence of 10–9 M hydrocortisone and 10–4 M cycloheximide. Cells were incubated in the presence of 10–9 M hydrocortisone for 48 h, and at pH 7.4 or 6.8 for the last 24 h. 10–4 M cycloheximide was added for the last 24 h. Apical membrane NHE3 protein abundance was measured by biotinylation followed by precipitation with streptavidin and Western blotting with anti-NHE3 antisera. (a) Typical blot. (b) Summary of data. *P < 0.05. n = 6.