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Effect of media pH on NHE3 protein abundance in the presence of 10–9 M hydrocortisone and 10–4 M cycloheximide. Cells were incubated in the presence of 10–9 M hydrocortisone for 48 h, and at pH 7.4 or 6.8 for the last 24 h. 10–4 M cycloheximide was added for the last 24 h. NHE3 protein abundance was measured by Western blot. (a) Typical blot. (b) Summary of data. *P < 0.05. n = 6.
