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Effect of 10–9 M hydrocortisone and media pH on NHE3 mRNA abundance. Cells were incubated in the presence or absence of 10–9 M hydrocortisone for 48 h, and at pH 7.4 or 6.8 for the last 24 h. NHE3 mRNA abundance was measured by Northern blot. (a) Typical blot. (b) Summary of data. *P < 0.05. n = 5 (–HC/6.8), n = 6 (–HC/7.4, +HC/6.8, +HC/7.4).
