Figure 6.

Pathological changes in 4.1R^–/– tissues. Hematoxylin- and eosin-stained sections of (a) normal and (b) 4.1R^–/– spleen sections. Note the effacement of the splenic nodules by the dramatic expansion of the red pulp areas in 4.1R^–/– spleen. Prussian blue–stained (c) normal and (d) 4.1R^–/– spleen. Iron deposition is decreased in 4.1R^–/– spleen red pulp areas due to increased use by rapidly proliferating erythroid precursors. Prussian blue–stained (e) normal and (f) 4.1R^–/– liver. Hematopoietic foci (arrows) are frequent and liver iron deposition increased in 4.1R^–/– mice, reflecting increased destruction of 4.1-null red cells. Note that no iron deposition is seen in areas of proliferating erythroid precursors, i.e., liver hematopoietic foci (arrows). Prussian blue stains of (g) normal and (h) 4.1R^–/– kidney. Note the extensive deposition of iron in 4.1R^–/– proximal tubules (arrows) and Bowman's capsule (arrowhead), indicating severe intravascular hemolysis of 4.1R-null red cells. Bar, 100 μM.