Figure 7.

Expression of β-galactosidase activity encoded within the β-geo cassette of the targeted 4.1R gene. Frozen sections of the knockout mouse were fixed and stained with the chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-galactosidase, as described in Methods. Expression was observed in the following tissues: (a) hippocampus (b) cerebellum (c) kidney (d) heart (e) stomach, and (f) lung. A, alveoli; BT, bronchiolar tree; DCT, distal convoluted tubule; DG, dentate gyrus; G, glomerulus; GC, granule cells of the cerebellum; GE, gastric epithelium; PA, pulmonary artery; PCT, proximal convoluted tubules.