Table 3.
Theoretical and measured masses of OmpUs of 16 V. cholerae isolates
| Isolate | GT | |
Theoretical |
|
|
Measured |
|
|---|---|---|---|---|---|---|---|
| |
|
|
1st exp |
|
2nd exp |
||
| massa | Δb | massc | Δ refd | massc | Δ refd | ||
| 080025/EZ |
1 |
34656 |
0 |
34755 |
+ 6 |
34567 |
+ 12 |
| FFIVC130 |
1 |
34656 |
0 |
34742 |
- 6 |
34543 |
- 12 |
| FFIVC129 |
1 |
34657 |
+ 1 |
N.D.e |
|
N.D.e |
|
| FFIVC114 |
4 |
35595 |
+ 939 |
35683 |
+ 934 |
35506 |
- 951 |
| 080025/FE |
2 |
34584 |
- 72 |
34672 |
- 77 |
34482 |
- 73 |
| 080025/FI |
2 |
34584 |
- 72 |
34678 |
- 71 |
34508 |
- 47 |
| 080025/FL |
3 |
35566 |
+ 910 |
35656 |
+ 907 |
35469 |
+ 914 |
| 17/110/2006 |
6 |
33871 |
- 785 |
33975 |
- 774 |
33733 |
- 822 |
| 2/110/2006 |
5 |
34961 |
+ 305 |
35031 |
+ 282 |
34875 |
+ 320 |
| 080025/FR |
singleton |
34870 |
+ 214 |
34951 |
+ 203 |
34784 |
+ 229 |
| 080025/GE |
3 |
35566 |
+ 910 |
35670 |
+ 922 |
35501 |
+ 946 |
| FFIVC050 |
singleton |
33840 |
- 816 |
33924 |
- 824 |
33748 |
- 807 |
| FFIVC084 |
singleton |
34811 |
+ 155 |
34884 |
+ 136 |
34683 |
+ 128 |
| FFIVC137 |
singleton |
35709 |
+ 1053 |
35813 |
+ 1065 |
N.D.f |
|
| 4/110/2006 |
singleton |
34122 |
- 534 |
34198 |
- 550 |
33977 |
- 578 |
| 14/110/2006 | singleton | 34826 | + 170 | N.D.f | 34716 | + 161 |
aTheoretical mass of mature OmpU in Da.
bDifference in mass with theoretical mass of OmpU of isolate 080025/EZ, in Da.
cMean of peak masses obtained from 4 different MALDI spots.
dThe average of OmpU peak masses of strain 080025/EZ and FFIVC130 was set as reference.
eN.D.: not determined, as OmpT instead of OmpU was assigned as the major peak in the 30000 – 40000 m/z range.
fN.D.: not determined because of failed measurement.