Table 4.
Results of BLASTp search using OmpU of Vibrio cholerae O1 El Tor N16961 (calculated molecular mass 34655.65 Da) as query sequence
| Hit nr. | Mutations compared to OmpU N16961 | Theoretical mass (Da) | Strain | Serogroup | Serotype | Biotype | Origin | Year of isolation | ctxAB a | tcpA a | Epidemic (E) or non-epidemic strain (N) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
34656 |
N16961 |
O1 |
Inaba |
El tor |
Bangladesh |
1975 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1032 (5) |
O1 |
Ogawa |
El tor |
Mexico |
1991 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1044 (17) |
O1c |
|
|
Peru |
1991 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
4260B |
O139 |
|
|
Bangladesh |
1993 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1046 (19) |
O1c |
|
|
Peru |
1995 |
ctxA+,ctxBf |
tcpA+ |
E |
| 1 |
|
34656 |
CP1047 (20) |
O1c |
|
|
Peru |
1995 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1033 (6) |
O1c |
|
|
Mexico |
2000 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CIRS101 |
O1 |
Inaba |
El tor |
Bangladesh |
2002 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1037 (10) |
O1 |
|
|
Mexico |
2003 |
ctxA+,ctxB-f |
truncated |
E |
| 1 |
|
34656 |
CP1040 (13) |
O1c |
|
|
Zambia |
2004 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1041 (14) |
O1 |
Ogawa |
El tor |
Zambia |
2004 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1030 (3) |
O1c |
|
|
Mexico |
2008 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
HC-06A1
e
|
O1 |
Ogawa |
El tor |
Haiti |
2010 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1042 (15) |
O1 |
Ogawa |
El tor |
Thailand |
2010 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1048 (21) |
O1 |
Ogawa |
El tor |
Bangladesh |
2010 |
ctxAB+ |
tcpA+ |
E |
| 1 |
|
34656 |
CP1050 (23) |
O1c |
|
|
Bangladesh |
2010 |
ctxAB+ |
tcpA+ |
E |
| 2b |
|
34656 |
M66-2 |
O1 |
- |
- |
Indonesia |
1937 |
ctxA+,ctxB-f |
tcpA+ |
E |
| 2 |
|
34656 |
MAK 757 |
O1 |
Ogawa |
El Tor |
Indonesia |
1937 |
ctxAB+ |
tcpA+ |
E |
| 2 |
|
34656 |
V52 |
O37 |
|
|
Sudan |
1968 |
ctxAB+ |
tcpA+ |
E |
| 2 |
|
34656 |
RC9 |
O1 |
Ogawa |
El Tor |
Kenya |
1985 |
ctxAB+ |
tcpA+ |
E |
| 2 |
|
34656 |
BX 330286 |
O1 |
Inaba |
El Tor |
Australia |
1986 |
ctxAB+ |
tcpA+ |
E |
| 2 |
|
34656 |
MO10 |
O139 |
|
|
India |
1992 |
ctxAB+ |
tcpA+ |
E |
| 2 |
|
34656 |
MJ-1236 |
O1 |
Inaba |
El Tor |
Bangladesh |
1994 |
ctxAB+ |
tcpA+ |
E |
| 2 |
|
34656 |
B33 |
O1 |
Ogawa |
El Tor |
Mozambique |
2004 |
ctxAB+ |
tcpA+ |
E |
| 3 |
F287I |
34622 |
unknown |
unknown |
|
El tor |
|
|
unknown |
unknown |
unknown |
| 4 |
G325D |
34714 |
CP1038 (11) |
O1 |
Ogawa |
El tor |
Zimbabwe |
2003 |
ctxAB+ |
tcpA+ |
E |
| 5 |
E290K, V324A, 325S |
34657 |
RC27 |
O1 |
|
Classical |
Indonesia |
1991 |
truncated |
truncated |
N |
| 5 |
E290K, V324A, 325S |
34657 |
O395 |
O1 |
Ogawa |
Classical |
India |
1965 |
ctxAB+ |
truncated |
N |
| 7 |
10 mut |
34598 |
BJG-01 |
non-O1d |
|
|
|
|
ctxA+,ctxB-f |
unknown |
N |
| 8 |
9 del , 13 mut |
33840 |
HE-25 |
non-O1d |
|
|
Haiti |
2010 |
ctxAB - |
tcpA - |
N |
| 9 |
9 del, 13 mut |
33840 |
AM-19226 |
O39 |
|
|
Bangladesh |
2001 |
ctxAB - |
tcpA - |
N |
| 10 | 7 del, 18 mut | 33911 | RC385 | O135 | USA | 1998 | ctxAB - | tcpA - | N |
actxAB and tcpA genes were identified by blastx search of whole genome sequences using ctxAB and tcpA of strain N16961 as query sequences.
bHit nr. 2 represents OmpU identical to hit nr. 1 except for nine additional N-terminal residues resulting from a wrongly identified translation start.
cPresumed serotype O1 based on sequence similarity with O-antigen biosynthesis genes VC0241 to VC0244A from N16961.
dPresumed serotype non-O1/O139, based on lack of sequence similarity with O-antigen biosynthesis genes VC0241 to VC0244A from N16961 and O139. Accession: AB012956 bp 22084–24660 wbfH/wbfI/wbfJ ).
eThis strain represents also 44 other Vibrio cholerae O1 El Tor Ogawa isolates from same outbreak with identical OmpU sequence and toxigenicity genes.
fNo ctxB similar to ctxB of N16961 (locus_tag;VC1456). Presence of another variant of ctxB cannot be excluded.