Figure 4.

Palladium-mediated conversion of prodrugs 5a,p,b into gemcitabine (1) in pancreatic cancer BxPC-3 cells. Experiments: 0.1% (v/v) DMSO (untreated cell control, in gray); 0.67 mg/mL of Pd⁰-resins (negative control, in black); gemcitabine (100 nM, positive control, in red); prodrug 5a,p,b (100 nM, negative control, in blue); and 0.67 mg/mL of Pd⁰-resins + prodrug 5a,p,b (100 nM, BOOM activation, in green). Cell viability was measured at day 5 using PrestoBlue reagent. Error bars: ± SD from n = 3.