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Bioorthogonally activated toxigenic effect in cancer cell culture: (a) BxPC-3 and (b) Mia PaCa-2 cells. Dose–response study: 0.1% (v/v) DMSO (untreated cell control, in gray); 0.67 mg/mL Pd0-resins (negative control, in black); gemcitabine (positive control, in red); prodrug 5a,p (negative control, in blue); and 0.67 mg/mL of Pd0-resins + prodrug 5a,p (BOOM activation, in green). Cell viability was measured at day 5 using PrestoBlue reagent. Error bars: ± SD from n = 3.
