Figure 3.
(a–g) Exogenous expression of β1C in NRP152 and CHO cell transfectants. Stable cell transfectants expressing β1C were generated using a tetracycline-regulated expression system. NRP152-β1C or CHO-β1C stable cell transfectants were cultured for 72 h, either in the absence (a and c) or in the presence (b and d) of 1 μg/ml tetracycline and analyzed by FACS® using TS2/16, MAB to human β1 integrin, or 12CA5 as a negative control, followed by FITC-goat anti–mouse IgG. Fluorescence intensity is expressed in arbitrary units. FACS® analysis of a representative β1C clone is shown. CHO cells were transiently transfected using pBJ1-β1C (e), or pBJ1-β1A (f), or pBJ-1 vector (g), and after 44 h, cells were stained and analyzed as described above. Thick line, TS2/16; thin line, 12CA5. MAB, monoclonal antibody.