Extended Data Figure 6. Electronic gating strategy for analysing the complexity of subcellular apoptotic particles generated ex vivo and in vivo.

a, Flow cytometry analysis showing electronic gating strategy used to distinguish annexin V^high, 7-AAD^low subcellular particles generated from primary mouse thymocytes induced to undergo apoptosis via dexamethasone treatment. Subcellular apoptotic particles with high complexity (SSC high) or low complexity (SSC low) are gated as shown. b, Flow cytometry analysis showing electronic gating strategy used to distinguish different subsets of apoptotic cell-derived particles generated in the thymus of mice injected intraperitoneally with dexamethasone (6 h). Annexin V^high, 7-AAD^low, CD4/CD8^intermediate particles were initially selected and subsequently gated based on forward scatter (FSC, indicative of cell size). Apoptotic particles of interest (as indicated) are therefore defined as Annexin V^high, 7-AAD^low, CD4/CD8^intermediate and FSC^{low/intermediate}.