Figure 4.

nNOS-positive Lis1^+/− parasympathetic neurons (PPNs) have migratory errors. A,B: On E13.5 both Lis1^+/+ (A, $d_{\mathit{PPN}}^{+/+}$) and Lis1^+/− (B, $d_{\mathit{PPN}}^{+/-}$) PPNs formed elongated nuclei. C,D: By E14.5 the intermediolateral sacral horn (ILS) contained PPNs in both genotypes. Ventrally positioned nNOS-positive SPNs (arrows) with dorsally oriented processes were present only in Lis1^+/− spinal cords (enlargement in I). E–H: E17.5 (E) and P0 (G) Lis1^+/+ PPNs are similar, whereas this cell group in Lis1^+/− mice (F,H) is more elongated and outliers (arrows) are detected ventrally. I–K: Lis1^+/− PPNs exhibit different arrangements of ectopically positioned cells: individual outliers with long processes oriented toward the ILS (I, enlarged from D), multiple groups of PPNs (J, shown at E14.5), and PPNs found all along the migratory pathway (K, shown at P0). Scale bar = 100 μm in A (applies to A–F) and G (applies to G,H); 50 μm in I–K.