Figure 5.

Choline acetyltransferase (ChAT)-positive parasympathetic preganglionic (PPNs) and somatic motor neurons (SMNs) do not completely separate in Lis1^+/− mice. A,B: On E13.5 the ChAT-positive cells in the ventral spinal cord form a continuous group in Lis1^+/+ and Lis1^+/− mice. C,E,G: Lis1^+/+ PPNs reach the sacral intermediolateral horn (ILS) by E14.5 (C, $d_{\mathit{PPN}-\mathit{SMN}}^{+/+}$). The distance between PPNs and SMNs continues to increase at E17.5 (E) and P0 (G). D,F,H: On E14.5 some Lis1^+/− PPNs (D, $d_{\mathit{PPN}-\mathit{SMN}}^{+/-}$) are found in the ILS with others scattered further ventrally. On E17.5 (F) and P0 (H) the Lis1^+/− PPNs remain more widespread and closer to the SMNs than wild-type PPNs. Scale bar = 100 μm in A (applies to A–F).