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Primary Antisera Used
| Antigen | Immunogen | Source and ID# | Species | Dilution |
|---|---|---|---|---|
| Choline acetyltransferase (ChAT) | Human placental enzyme | Chemicon (Temecula, CA); AB144P | Goat polyclonal | 1:300 |
| Neuronal nitric oxide synthase (nNOS) | Human C-terminal peptide sequence (aa 1419–1433) | Immunostar (Hudson, WI); 24287 | Rabbit polyclonal | 1:8,000–13,000 |
