Figure 5.

Evidence for increased Ca²⁺-mediated proteolysis following MCA occlusion/reperfusion in gsn^–/– mice. Gsn^+/+ and gsn^–/– mice were subjected to 2 h MCA occlusion and were sacrificed 1 h after reperfusion. Coronal brain sections were immunostained with antibodies against calpain-cleaved spectrin and MAP-2; additional sections were reacted with biotinylated horse anti–mouse IgG (upper panels). Note increased IgG immunoreactivity, increased spectrin immunoreactivity, and decreased MAP-2 immunoreactivity in the gsn^–/– mice. These micrographs are representative of five gsn^–/– and four gsn^+/+ mice examined. MAP-2, mitogen-activated protein-2.