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. 1999 Feb 1;103(3):355–363. doi: 10.1172/JCI5121

Figure 3.

Figure 3

Surface expression and dominant–negative function of ICD mutant CD95 alleles. (a) Expression. Human embryonic kidney 293T cells were transfected with pCDNA3 expression vectors encoding WT or mutant CD95. CD95 expression was analyzed by flow cytometry using the anti-CD95 MAB, UB2. The percent of CD95-positive cells for each transfection is shown. (b) Function. CD95-positive Jurkat T cells were cotransfected with the same panel of expression vectors as in a and with the reporter gene, RSV-Luc. The cells were separated into two aliquots and cultured with either the agonistic anti–CD95 antibody CH11 (50 ng/ml) or an isotype control antibody 24 h after transfection. Luciferase expression was assayed at 48 h and percent viability was calculated by the following formula: (luciferase activity of CH11/luciferase activity of control antibody) × 100. The data shown are the mean results of two independent experiments. WT, wild type; RSV, Rous sarcoma virus.