Figure 5.

Death domain missense mutations (P7 and P8) distort its α-helical structure. The peptide sequence for wild-type CD95 (residues 223–273) and the same regions from P2, P7, and P8 mutant alleles were analyzed by secondary structure predictive models (see Methods). The regions of each sequence that were predicted to adopt an α-helical conformation (H) are indicated above the corresponding region of the amino acid sequence. Dashes (–) represent areas predicted not to form an α-helix. The stippled boxes highlight the regions predicted to be altered by the mutations.