Figure 4. Developmental ontogeny and genetic dissection of spinal microcircuits.
(a) Ontogeny of spinal neurons. “Excit.”: excitatory neurons. “Inhib.”: inhibitory neurons. “Sst”: somatostatin; “Tac2”:tachykinin 2; “NPY1r”: neuropeptide Y receptor 1; “GRP”: gastrin-releasing peptide; “GRPR”: GRP receptor; “Galr1”: galanin receptor 1; “Dyn”: dynorphin; “N/OFQ”: nociceptin/orphanin FQ; “Enk”: enkephalin; “ChAT”: choline acetyl transferase; “Sstr2”: somatostatin receptor 2; “Gal”: galanin. (b) Schematic depicting the intersectional genetic manipulation. The top is the intersectional reporter mice: Tau-FSTOPF-LSTOPL-X. “F”: the Flpo recominase recognition sequence; “L”: the Cre recombinase recognition sequence. (c) A list of intersectional reporters. “DREDD”: “designer receptor exclusively activated by a designer drug”; “ChR2”: channelrhodopsin; “HR”: halorhodopsin.