Table 1. RT-PCR primers.
Gene name |
Primers | Location in mRNA |
Accession Number |
Length | Location in DNA |
---|---|---|---|---|---|
CGRP | GTGTCACTGCCCAGAAGAGATC | 358–379 | NM_001033955.1 | 101 | 3772–3793 |
CAAAGTTGTCCTTCACCACACC | 438–459 | 3852–3873 | |||
GAPDH | ATGACATCAAGAAGGTGGTG | 837–856 | NM_017008.3 | 176 | 3325–3344 |
CATACCAGGAAATGAGCTTC | 994–1013 | 3482–3501 | |||
RAMP1 | ACTGGGGAAAGACCATAGGGAG | 212–233 | NM_031645.1 | 229 | 20481–20500 |
AGTCATGAGCAGTGTGACCGTA | 420–441 | 46326–46347 | |||
CLR | ATTGTGGTGGCTGTGTTTGCA GAG | 1126–1149 | NM_012717.1 | 141 | 78843–78866 |
TGTCTGAGCTGATCCAGCAGTT GT | 1244–1267 | 80720–80739 | |||
RCP | GAACTTGAACGCCATCACCT | 134–153 | NM_053670.3 | 172 | 12773–12792 |
GGATCTCAACAGCGGTCATT | 287–306 | 24220–24239 |