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. Author manuscript; available in PMC: 2014 Jul 2.
Published in final edited form as: Headache. 2011 May;51(5):674–692. doi: 10.1111/j.1526-4610.2011.01882.x

Table 2.

Expression of CGRP-related genes in a rat model of chronic migraine.

Trigeminal Ganglion
Naïve 10 µL PBS 10 µL IS 10 µL PBS vs IS 20 µL PBS 20 µL IS 20 µL PBS vs IS
MALES CGRP 1.04 +/− 0.16 0.51 +/− 0.11** 0.43 +/− 0.08** NS 0.43 +/− 0.02* 0.62 +/− 0.07* NS
RAMP1 1.01 +/− 0.06 0.41 +/− 0.04** 0.47 +/− 0.14* NS 1.90 +/− 0.87 2.22 +/− 0.67 NS
CLR 1.03 +/− 0.11 0.10 +/− 0.05** 0.18 +/− 0.04** NS 3.08 +/− 0.54* 2.55 +/− 0.46** NS
RCP 1.06 +/− 0.14 0.48 +/− 0.10** 1.18 +/− 0.24 (P = 0.04) 1.82 +/− 0.28* 7.61 +/− 3.26** NS
FEALES CGRP 1.13 +/− 0.21 1.52 +/− 0.87 2.26 +/− 1.04 NS 0.80 +/− 0.02 0.87 +/− 0.16 NS
RAMP1 1.05 +/− 0.16 1.68 +/− 0.60 3.20 +/− 1.39 NS 1.16 +/− 0.22 1.38 +/− 0.22 NS
CLR 1.08 +/− 0.17 4.23 +/− 1.30 3.39 +/− 1.79 NS 4.14 +/− 0.45** 4.26 +/− 0.36** NS
RCP 1.14 +/− 0.24 2.75 +/− 0.59* 3.48 +/− 0.78* NS 3.43 +/− 0.82* 3.89 +/− 0.60** NS
Medulla
Naïve 10 µL vehicle 10 µL IS 10 µL PBS vs IS 20 µL vehicle 20 µL IS 20 µL PBS vs IS
FEMALES CGRP 1.04 +/− 0.13 1.73 +/− 0.51 1.35 +/− 0.36 NS 0.48 +/− 0.08* 0.52 +/− 0.05* NS
RAMP1 1.01 +/− 0.05 1.07 +/− 0.25 0.60 +/− 0.16 NS 1.95 +/− 0.46 0.73 +/− 0.03** NS
CLR 1.29 +/− 0.31 0.27 +/− 0.14* 0.17 +/− 0.11** NS 0.54 +/− 0.10 2.93 +/− 1.73 NS
RCP 1.05 +/− 0.13 1.34 +/− 0.38 0.76 +/− 0.21 NS 0.78 +/− 0.14 0.94 +/− 0.16 NS
FEMALES CGRP 1.08 +/− 0.18 0.71 +/− 0.15 0.99 +/− 0.28 NS 0.673 +/− 0.12 0.56 +/− 0.11* NS
RAMP1 1.05 +/− 0.14 1.63 +/− 0.32 2.56 +/− 0.53* NS 1.49 +/− 0.13 0.99 +/− 0.12 (P = 0.04)
CLR 1.07 +/− 0.23 18.78 +/− 10.21** 11.46 +/− 3.56** NS 14.99 +/− 2.38* 14.31 +/− 2.10** NS
RCP 1.62 +/− 0.81 5.44 +/− 1.46* 6.26 +/− 1.26** NS 7.11 +/− 0.10* 7.51 +/− 1.27** NS

Numbers indicate relative expression values for expression of CGRP, RAMP1, CLR, and RCP in the trigeminal ganglion and medulla in male and female rats in all experimental groups as measured by qRT-PCR using Δ ΔCt analysis. GAPDH was used as the internal control. Naïve sex-matched animals were used as subject control for normalization. Male naïve group, n=6; 10µl PBS group, n = 5; 10µl IS group, n = 5; 20µl PBS group, n = 3; 20µl IS group, n = 5; Female naïe group, n=6; 10µl PBS group, n = 9; 10µl IS group, n = 7; 20µl PBS group, n = 3, 20µl IS group, n = 5. Bolded, outlined cells marked with * indicate P <0.05 when compared with naïve control and ** indicate P <0.005 when compared with naïve control. PBS vs IS column compares IS treated groups with their equal volume controls. The P value is given when results are significant and NS given when not significant. Comparisons with significant differences from baseline are indicated by bold font and outline boxes.