Skip to main content
. Author manuscript; available in PMC: 2014 Jul 2.
Published in final edited form as: DNA Repair (Amst). 2011 Jan 20;10(3):338–343. doi: 10.1016/j.dnarep.2010.12.005

Fig. 1.

Fig. 1

PARP inhibitors impair survival in response to challenge with MMS, but do not impair gene conversion, while overexpression of the BRCT domain of PARP-1 has the opposite effect. (A) Survival of indicated cell lines in response to MMS induced damage. Error bars indicate the SEM of three experiments. (B) Frequency of gene conversion events in respective cell lines. Error bars indicate the SEM of 2–4 cell lines cultured independently. (C) Schematic of BRCT overexpression construct. (D) Survival of indicated cell lines in response to MMS induced damage. Error bars indicate the SEM of three experiments. (E) Frequency of gene conversion events in respective cell lines. Error bars indicate the SEM of 2–4 cell lines cultured independently. (F) Western blot showing expression of the BRCT overexpression construct in multiple independent DT40 cell lines which fluoresced green, stained with anti-FLAG (Sigma).