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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Mol Microbiol. 2014 Jun 4;93(1):183–198. doi: 10.1111/mmi.12650

Figure 8. Nonfunctional sfp gene results in large quantities of recovered vesicles.

Figure 8

Cells from strains (A) 168 and (B) 3610 were labeled with 14C PA and the radioactivity of each fraction measured (cells, FT, supernatant, and vesicle pellet). (C) Strain 168 vesicle pellet contained significantly more radioactivity than that of strain 3610 (p<0.01 t-test, n=3). Vesicle pellets of B. subtilis strains 168 and AD3610 harboring (D) nonfunctional sfp, contain significantly higher radioactivity in the vesicle pellet than that of functional sfp strains 3610, RL3090, and SL3610 (p<0.01 one-way ANOVA, n=3). (E) Density gradient of 14C PA labeled vesicles indicating the location and density of vesicles from each strain. (F) Vesicle pellets from strains 168 and RL2663 contain significantly more radioactivity than that of the surfactin-producing strain 3610 (p<0.01 one-way ANOVA, n=3).