Figure 4.

Effect of NHE-1 and FXR inhibition in suppression of growth of nude mouse xenografts. The mice were treated with 5 mg/kg amiloride, 50 mg/kg guggulsterone, or a combination of both (as the same individual dose) for 2 days before they were subcutaneously injected with 3 million SKGT-4 cells. After that, the mice were continuously given oral 5 mg/kg amiloride, 50 mg/kg guggulsterone, or a combination of both (as the same individual dose) daily. Tumor formation and growth were monitored daily. At the end of the experiments, the mice were euthanized and the tumor xenografts were resected, fixed in 4% paraformaldehyde, weighed, and photographed.