Figure 6.

Characterisation of fibroblastic cells with respect to cell-cell adhesion and localisation and cytoskeletal attachment of E-cadherin. (A) Influence of forced E-cadherin expression on cell-cell adhesion, as measured by dissociation assay, in epithelial TrE-ep5 and fibroblastic TrE-fib cells (p-values obtained by Student’s t-test; NS, not significant). (B) Immunofluorescence micrographs showing localisation of E-cadherin, β-catenin and γ-catenin in Tr-ep cells and in TrE-fib cells with and without dox treatment. For analysis of E-cadherin, cells were not permeabilised before staining; thus, only cell surface-bound E-cadherin is visualised. (C) Percentage of E-cadherin detected after extraction of membrane lipids with Triton X-100 in Tr-ep, TrE-ep1 and TrE-fib cells with and without dox treatment as indicated. n, number of independent experiments; p, p-value in Student’s t-test for comparison with results for Tr-ep cells. Error bars, SEM.