Figure 7.

Properties of Tr-ep cells expressing dominant-negative E-cadherin. (A) Dox-induced expression of the E-cadherin ‘WV’ mutant construct, detected as an increase in total E-cadherin levels (using mAb HECD-1 staining of non-permeabilised cells), in flow cytometry. (B) Effects of E-cadherin mutant expression on cell-cell adhesion as measured in dissociation assay (see Materials and methods for details). TrGFP-ep cells, Tr-ep cells expressing GFP only from the TO/IRES-GFP construct, were used as control. p, p-value in Student’s t-test for comparison with TrGFP-ep cells. Error bars, SEM.