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Expression of mutant E-cadherin fails to promote c-erbB2-induced EMT at high cell density. (A) Photomicrographs of TrEWV-ep cells grown at low density for two passages with or without dox and NGF treatment as indicated. Note that all micrographs are taken at the same magnification. (B) Expression of the mesenchymal marker vimentin in TrEWV-ep cells after 4 passages in the absence or presence of NGF and/or dox at low or high cell density, as measured by flow cytometry.
