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. 2014 Apr 23;45(1):439–447. doi: 10.3892/ijo.2014.2396

Figure 2.

Figure 2.

ATN-224 induces caspase-independent cell death. (A) Caspase 3 activity measured in SUDHL-4, SUDHL-8 and SUDHL-10 cells treated with vehicle or ATN-224 (EC50) for 24 h. (B) Immunoblots showing cytochrome c release from the mitochondria in SUDHL-4, SUDHL-8 and SUDHL-10 cells treated with vehicle or ATN-224 (EC50) for 24 h. C+N, cytosolic and nuclear fraction; M, mitochondrial loading control. Immunoblots showing actin demostrate similar loading. Immunoblots showing COX IV demostrate the integrity of the mitochondrial prep. All immunoblots are representative blots from three independent sample collections. (C) Cell viability in SUDHL-4, SUDHL-8 and SUDHL-10 cells treated with vehicle, 2 μM ZVAD-FMK (ZVAD), ATN-224 (EC50) or a combination of ZVAD-FMK and ATN-224 for 72 h. All values are mean ± SEM (n ≥3). *p≤0.05, significantly different from vehicle treated control cells.