Figure 3.

ATN-224 induces AIF release from the mitochondria. (A) Immunoblots showing AIF release from the mitochondria in SUDHL-4, SUDHL-8 and SUDHL-10 cells treated with vehicle or ATN-224 (EC₅₀) for 24 h. C+N, cytosolic and nuclear fraction; M, mitochondrial loading control. Immunoblots showing actin demostrate similar loading. Immunoblots showing COX IV demostrate the integrity of the mitochondrial prep. All immunoblots are representative blots from three independent sample collections. (B) Quantification of AIF release from the mitochondria. (C) Nuclear condensation in SUDHL-4, SUDHL-8 and SUDHL-10 cells treated with vehicle or ATN-224 (EC₅₀) for 24 h. All values are mean ± SEM (n ≥3). ^*p≤0.05, significantly different from ATN-224 treated control cells.