Table 1.
Primer pairs used to amplify the seven overlapping fragments of HVS-I with molecular details
| Amplicon size (bp) | Primer name | Primer range | Primer sequence 5′-3′ | Sequence information range (bp) | Reference |
|---|---|---|---|---|---|
|
170 |
L15422 H15555 |
15404-15422 15555-15574 |
CTCTTGCTCCACCATCAGC TTATATGCATGGGGCAAACC |
133 |
[124] |
|
219 |
L15511 H15692 |
15490-15511 15692-15709 |
ACTGTGCTATGTCAGTATCTCC TTGATGGTTTCTCGAGGC |
181 |
[124] |
|
246 |
L15574 H15780 |
15555-15574 15780-15801 |
GGTTTGCCCCATGCATATAA AAGTAAGAACCAGATGCCAGGT |
206 |
[126] |
|
148 |
L15764 H15873 |
15744-15764 15873-15892 |
CCCATACTAACGTGGGGGTTA TGTGTGATCATGGGCTGATT |
109 |
[126] |
|
129 |
L15857 H15945 |
15836-15857 15945-15965 |
ATTCTCGCAAATGGGACATC GCGGTCGTAGGTGAGTGATAG |
88 |
[126] |
|
137 |
L15892 H15991 |
15873-15892 15991-16010 |
AATCAGCCCATGATCACACA GGCATATTCCCTGAGAGCAG |
99 |
[126] |
| 154 | L15982 H16097 | 15963-15982 16097-16117 | CGCAACGGCACTAACTCTAA TGTCCTGAAACCATTGACTGA | 115 | [126] |