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. 2014 Jun 4;15:70. doi: 10.1186/1471-2202-15-70

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Copyright © 2014 Pfeiffer-Guglielmi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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FISH detection of GS mRNA in cortical neuronal cultures, 4 DIV. GS mRNA is prominent in neuronal cell bodies and colocalizes with MAP 2 in dendrites (a-c) and tau in axons. (d-f). Higher magnification (1 and 2) of the boxed areas in c and f reveals mRNA clusters in dendritic and axonal processes. FISH for GS mRNA combined with GAP 43 ICC reveals both signals in the axon, but colocalization in growth cones is not obvious (g-i). j-l Negative control applying sense probe in combination with MAP 2 ICC. Bar in a = 20 μm and applies to a-f and j-l. Bar in g = 10 μm and applies to g-i. Bar in 1 = 5 μm, bar in 2 = 10 μm.