Table 5.
Performance based on biomass concentration, lipid accumulation, and CO2 fixation of salinity-gradient culturing of Chlamydomonas sp. JSC4 with stepwise addition of sea salt at different increments
| Sea salt addition (% d-1) | Cultivation time under nitrogen depletion (days) | Biomass concentration (g L-1) | Lipid content (%) | Lipid productivity (mg L-1 d-1) | CO2 fixation rate (mg L-1 d-1) |
|---|---|---|---|---|---|
| 0.0 (batch) |
0 |
1.426 ± 0.008 |
15.8 ± 0.1 |
111 ± 0 |
1319 ± 6 |
| 3 |
2.232 ± 0.007 |
41.9 ± 1.8 |
159 ± 8 |
731 ± 3 |
|
| 5 |
2.467 ± 0.064 |
49.8 ± 2.3 |
152 ± 11 |
561 ± 16 |
|
| 0.5 |
0 |
1.471 ± 0.010 |
15.3 ± 0.3 |
101 ± 3 |
1243 ± 9 |
| 3 |
2.129 ± 0.004 |
59.4 ± 0.7 |
223 ± 2 |
706 ± 1 |
|
| 5 |
2.180 ± 0.042 |
63.7 ± 0.8 |
175 ± 3 |
515 ± 3 |
|
| 1.0 |
0 |
1.445 ± 0.021 |
15.7 ± 0.6 |
102 ± 5 |
1219 ± 19 |
| 3 |
2.044 ± 0.015 |
60.2 ± 2.5 |
217 ± 7 |
677 ± 5 |
|
| 5 |
2.082 ± 0.079 |
64.9 ± 2.7 |
170 ± 14 |
491 ± 20 |
|
| 1.5 |
0 |
1.436 ± 0.007 |
15.5 ± 0.6 |
100 ± 3 |
1211 ± 6 |
| 3 |
2.009 ± 0.001 |
53.7 ± 2.0 |
190 ± 7 |
665 ± 0 |
|
| 5 | 2.044 ± 0.090 | 59.4 ± 0.3 | 152 ± 6 | 482 ± 22 |
The values are the mean ± standard deviation of three replicated experiments. (Light intensity = 200 μmol m-2 s-1; CO2 aeration = 2.0%; CO2 flow rate = 0.05 vvm).