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. 2014 Jul 2;9(7):e101364. doi: 10.1371/journal.pone.0101364

Figure 3. Effects of βA42 on viral internalization and viral replication in MDCK cells as determined by quantitative RT-PCR.

Figure 3

In panels A, Phil82 IAV was pre-incubated with the indicated concentrations of βA42, followed by incubation of these samples for 45 min with MDCK cells as in figure 1. The cell supernatant and cell pellets were then harvested and viral RNA was extracted followed by PCR assay to determine the amount of virus present in cells and supernatant. βA42 significantly reduced the amount of virus taken up by cells after 45 min of infection. There was a trend to increased virus in the supernatant after 45 min of infection but this was not significant. Panel B shows the results of cell and supernatant assays after 24 hrs of infection. βA42 significantly reduced the amount of virus in both cell and supernatant at this time. * indicates p<0.05 compared with control. Results are mean±SEM of three experiments.