Figure 9. Effects of βA42 on interactions of IAV with monocytes: viral protein synthesis (panel A), viral uptake (panel B) and virus-induced cytokine generation (panel C).

Adherent peripheral blood monocytes were infected with Phil82 IAV alone or Phil82 IAV treated with the indicated doses of βA42. In panel A the presence of viral nucleoprotein in the cells was tested as in figure 1 after 24 hours of incubation. * indicates significant reduction compared to virus alone (p<0.03; n = 4 separate blood donors). In panel B the ability of monocytes to take up fluorescently labeled IAV after a 45 min incubation was tested. Increased uptake (p<0.05; n = 4) is indicated by *. In panel C the monocytes were incubated with IAV+/−peptide for 24 hrs and then TNF or IL-6 release into the supernatant was measured by ELISA. IAV alone stimulated increased cytokine production by the cells (see Results section). IL-6 production was significantly reduced by pre-incubation of the virus with βA42 (p<0.02; n = 4; indicated by *). TNF production was not reduced by βA42 (n = 8).