Table 2.
LC-MS/MS Analysis of Serum Vitamin D Metabolites in Several CYP Knockout Mice
| Cyp2r1/Cyp27a1/CYP24a1 | 25-OH-D3, nmol/La | 24,25-(OH)2D3, nmol/La | 25-OH-D3-26, 23-Lactone, nmol/La | 25-OH-D3 to 24, 25-(OH)2D3 ratioa |
|---|---|---|---|---|
| ++/++/++ | 42.2 ± 3.4 | 28.9 ± 2.2 | 13.5 ± 1.4 | 1.5 ± 0.1 |
| +−/++/++ | 34.7 ± 3.3 | 25.5 ± 2.6 | 9.2 ± 2.1 | 1.4 ± 0.0 |
| −−/++/++ | 16.8 ± 2.5 | 13.5 ± 1.6 | 4.0 ± 0.9 | 1.2 ± 0.1 |
| ++/−−/++ | 176.3 ± 28.3 | 107.7 ± 6.4 | 42.1 ± 6.9 | 1.6 ± 0.2 |
| −−/−−/++ | 28.1 ± 3.8 | 25.8 ± 5.1 | 5.9 ± 0.9 | 1.1 ± 0.1 |
| ++/++/+− | 37.5 ± 3.6 | 19.2 ± 2.2 | 9.5 ± 0.8 | 2.0 ± 0.1 |
| ++/++/−− | 256.6 ± 23.1 | 2.9 ± 0.3 | 0.3 ± 0.0 | 87.6 ± 9.2 |
a
Mean ± SD, n = 3–8 animals.