Figure 3. Overexpression of CXCL12 induces a diminishment of PARP-1 activity.
Poly(ADP-ribosyl)ation level in islet cells, wt and #1 cells after treatment with IC50 hydrogen peroxide determined by immunoblot (A, top panel) and by immunofluorescent analysis in wt and #1 cells (B) with anti-PAR antibody. The PARP-1 protein level in wt and #1 cells was determined by immunoblot analysis with anti-PARP-1 antibody (A, lower panel). (C) mRNA levels of PARP-1 and PARG in wt and #1 control cells and cells after 1 h of hydrogen peroxide treatment followed by 5 h of recovery in the cell culture media. The mRNA levels were determined by RT-qPCR and presented in the graphs depicting the changes in mRNA levels relative to β-actin. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).