Table 1. Peptide Sequences, Activity, and Affinity to the HSL2 Receptor.
| Peptide | Amino Acid Sequence | EC50 (nM) | IC50 (nM) |
|---|---|---|---|
| EPIP | FGYLPKGVPIPPSAPSKRHN | 333 | >10,000 |
| PIP | PIPPSAPSKRHN | 33 | >10,000 |
| PIP-o4 | PIPoSAPSKRHN | 33 | ND |
| PIPPo | PIPPSAoSKRHN | 1 | 100 |
| PIP-o1;7 | oIPPSAoSKRHN | 1 | ND |
| PIP-o3;7 | PIoPSAoSKRHN | 1 | 33 |
| PIPPo-∆N | PIPPSAoSKRH | >10,000 | >10,000 |
| VPIPPo | VPIPPSAoSKRHN | 1 | 33 |
| acri-PIPPo | acr-VPIPPSAoSKRHN | 100 | ND |
| CLV3* | RTVoSGoNPLHHH | >10,000 | ND |
| [Ara3]CLV3* | [Ara3]-RTVoSGoNPLHHH | >10,000 | ND |
Amino acid sequences of peptides used in this study are indicated in single-letter amino acid abbreviations with “o” for hydroxyproline. EC50, concentrations required to stimulate half-maximal oxidative burst in N. benthamiana leaves expressing HSL2; IC50, concentration required for 50% reduction of acri-PIPPo binding to HSL2; >10,000, no half-maximal effect on ox-burst or inhibition of binding even at 10 µM of peptides applied; ND, not determined. Asterisks indicate that the same results were obtained for HAE-expressing tissue.