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. 2014 May 16;26(5):1938–1948. doi: 10.1105/tpc.114.125807

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© 2014 American Society of Plant Biologists. All rights reserved.

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Figure 5. — Subcellular Localization of At4g19010.

(A) Alignment of the C-terminal regions of At4g19010 and its orthologs in cacao tree (Theobroma cacao), willow (Salix arbutifolia), poplar (Populus trichocarpa), cucumber (Cucumis sativus), strawberry (Fragaria vesca), peach (Prunus persica), chickpea (Cicer arietinum), tomato (Solanum lycopersicum), grape vine (Vitis vinifera), maize (Zea mays), rice (Oryza sativa), Brachypodium distachyon, and Selaginella moellendorffii. The tripeptides corresponding to consensus peroxisomal targeting sequence-1 are highlighted in red. Identical residues are shaded in black and similar ones in gray. Dashes symbolize gaps introduced to maximize alignment.

(B) Left panel, green pseudocolor of GFP fused to the N terminus of At4g19010; center panel, red pseudocolor of peroxisomal marker RFP-tagged 3-keto-acyl-CoA thiolase 2 (KAT2); right panel, overlay.

(C) Left panel, green pseudocolor of GFP fused to the N terminus of At4g19010; center panel, red pseudocolor of mitochondrial marker RFP-tagged isovaeryl-CoA dehydrogenase (IVDH); right panel, overlay.