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. 2014 May 13;26(5):1967–1980. doi: 10.1105/tpc.114.125047

Figure 2.

Figure 2.

JA Stabilizes MYC2, MYC3, and MYC4 Protein Levels.

(A) Immunoblot analysis of MYC2-GFP and actin protein levels in 35S:MYC2-GFP transgenic plants. Seven-day-old seedlings were treated with 50 μM CHX and 50 μM JA or mock (dimethylformamide) and harvested at the indicated times.

(B) Immunoblot analysis of MYC2-GFP and actin protein levels in 35S:MYC2-GFP transgenic plants in wild-type and coi1-16 backgrounds. Seven-day-old seedlings were treated with 50 μM CHX and harvested at the indicated times.

(C) Immunoblot analysis of MYC3-HA, MYC4-GFP, and actin proteins levels in 35S:MYC3-HA and 35S:MYC4-GFP transgenic plants. Seven-day-old seedlings were treated with 50 μM CHX and 50 μM JA or mock (dimethylformamide) and harvested at the indicated times.

Protein molecular mass is shown on the right. These experiments were repeated three times with similar results.