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. 2014 May 13;26(5):1967–1980. doi: 10.1105/tpc.114.125047

Figure 5.

Figure 5.

phyB Is Required for MYC2 Stability.

(A) Immunoblot analysis of MYC2-GFP and actin protein levels in 35S:MYC2-GFP transgenic plants in wild-type and phyB backgrounds (pB). Seedlings were grown for 4 d in continuous red light (Rc), white light/dark cycles (L/D, 16/8 h), or in the dark (D) and harvested.

(B) JA stabilizes MYC2 in the phyB background. Immunoblot analysis of MYC2-GFP and actin protein levels in 35S:MYC2-GFP transgenic plants in phyB background. Seven-day-old seedlings were treated with 50 μM CHX and 50 μM JA or mock (dimethylformamide) and harvested at the indicated times.

(C) Immunoblot analysis of MYC3-HA, MYC4-GFP, and actin protein levels in 35S:MYC3-HA and 35S:MYC4-GFP transgenic plants in wild-type and phyB backgrounds (pB). Seedlings were grown for 4 d in continuous red light (Rc), white light/dark cycles (L/D, 16/8 h), or in the dark (D) and harvested.

Protein molecular mass is shown on the right side. These experiments were repeated three times with similar results.