Figure 1.

Comparison of Deduced Amino Acid Sequences of Cm-BBX24 with BBXs from Other Plant Species and Expression Patterns of Cm-BBX24 in Chrysanthemum.

(A) Alignment of deduced amino acid sequences of Cm-BBX24 with other plant BBXs, with conserved amino acids shaded in different colors. The stars before Cm-BBX24 indicate the deduced protein sequence from chrysanthemum and melon (Cucumis melo), respectively. Red lines indicate two conserved B-box domains (B1 and B2), and the asterisks indicate the Zn²⁺-ligating conserved Cys, His, and Asp residues present in the B-box.

(B) Phylogenetic analysis of Cm-BBX24 and eight other structure group IV BBXs from Arabidopsis. Bootstrap values indicate the divergence of each branch and the scale indicates branch length.

(C) Expression of Cm-BBX24 in different tissues at various developmental periods. 1, young plant; 2, mature plant before differentiation; 3, initial period of flower bud differentiation; 4, visible capitulum; 5, initial flowering; 6, flowering; AB, apical buds; L, leaves; S, stems; R, roots; FB, flower buds; F, flowers. Ubiquitin was used as the control. Error bars indicate sd (n = 3). Significant differences were analyzed by Duncan’s multiple range test (P < 0.05).

(D) Expression of Cm-BBX24 under cold, dehydration and salt stress conditions. Error bars indicate sd (n = 3).

[See online article for color version of this figure.]