Table 2. Concentration of GAs in the Leaves of Cm-BBX24-OX/RNAi and Wild-Type Chrysanthemum Lines under SD and LD.
Daylength | Lines |
GA Content (ng⋅g−1
FW) |
||||||
---|---|---|---|---|---|---|---|---|
GA1 | GA4 | GA19 | GA24 | GA20 | GA3 | GA8 | ||
SD | Cm-BBX24-RNAi | 1.73 ± 0.17 a | 0.33 ± 0.04 a | 1.10 ± 0.16 a | 0.31 ± 0.07 a | 0.35 ± 0.03 a | 0.34 ± 0.05 a | 0.80 ± 0.23 a |
Wild type | 1.64 ± 0.19 a | 0.22 ± 0.05 b | 0.94 ± 0.17 a | 0.29 ± 0.05 a | 0.36 ± 0.09 a | 0.38 ± 0.09 a | 0.60 ± 0.06 a | |
Cm-BBX24-OX | 1.46 ± 0.13 a | 0.23 ± 0.04 b | 1.02 ± 0.14 a | 0.21 ± 0.06 a | 0.37 ± 0.06 a | 0.42 ± 0.09 a | 0.56 ± 0.23 a | |
LD | Cm-BBX24-RNAi | 1.45 ± 0.26 a | 0.34 ± 0.03 a | 1.40 ± 0.04 a | nd | 0.43 ± 0.04 a | 0.21 ± 0.03 a | 1.04 ± 0.34 a |
Wild type | 0.62 ± 0.11 b | 0.18 ± 0.01 b | 0.67 ± 0.07 b | nd | 0.28 ± 0.07 b | 0.19 ± 0.04 a | 0.69 ± 0.18 a | |
Cm-BBX24-OX | 0.37 ± 0.08 c | 0.12 ± 0.01 c | 0.58 ± 0.05 b | nd | 0.32 ± 0.02 b | 0.20 ± 0.02 a | 0.94 ± 0.07 a |
Cm-BBX24-OX, the wild type, Cm-BBX24-RNAi, and three different chrysanthemum lines. SD, 8-h SD; LD, 16-h LD. Significant differences were determined by Duncan’s multiple range test (P < 0.05, n = 3), and lowercase letters, a to c, indicate significant differences between wild-type and transgenic plants under SD or LD condition. nd, endogenous GA not detected, deuterated GA internal standard present. GA5, GA9, GA29, GA34, and GA51 were not detected.