Figure 1.

A Dominant-Negative RABG3f Mutant Protein Induces the Formation of Enlarged PVCs, Affects Vacuole Morphology, and Inhibits Vacuolar Trafficking.

(A) Subcellular localization of GFP-RABG3f, GFP-RABG3f^Q67L, and GFP-RABG3f^T22N expressed under a Dex-inducible promoter and working model of RABG3f localization in plant cells.

(B) YFP-RABG3f was crossed into a PVC marker line to analyze its localization.

(C) to (E) Confocal microscopy images of the PVC marker YFP-ARA7 (C), the tonoplast marker YFP-VAMP711 (D), and the lytic vacuolar cargo marker spL-RFP (E) in 6-d-old RABG3f^T22N seedlings grown in the absence or presence of 10 μM Dex.

Bars = 10 μm in (A) to (E).