Figure 7.

Endogenous Storage Proteins Were Mis-Sorted into the Extracellular Space in mon1-2 Mutant Seeds.

Immunogold electron microscopy analysis was performed in wild-type ([A] and [C]) and mon1-2 mutant ([B] and [D]) seeds using antibodies against the seed storage proteins 12S globulin ([A] and [B]) or 2S albumin ([C] and [D]). Arrowheads indicate PSVs that were positive for both antibodies in wild-type and mutant seeds, while arrows indicate extracellular space positive for labeling only in mutant seeds (arrows in [B] and [D]). e, extracellular space. Bars = 500 nm.