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. 2014 May 23;26(5):2129–2142. doi: 10.1105/tpc.114.126037

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© 2014 American Society of Plant Biologists. All rights reserved.

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Figure 2. — SAUR19 Interacts with D-Clade PP2Cs.

(A) Yeast two-hybrid interaction between SAUR19 and three D-clade PP2Cs. Serial dilutions of yeast cells harboring a P_GAL:HIS3 reporter were spotted onto selective media lacking histidine and supplemented with 10 mM 3-aminotriazole.

(B) Immunoblot analysis of soluble (S), lower phase-enriched other membrane (OM), and upper phase-enriched PM fractions from 7-d-old GFP-SAUR19[35S:PP2C-D1] seedlings. Five micrograms of each fraction were loaded onto the gel.

(C) Coimmunoprecipitation of GFP-SAUR19 and PP2C-D1 from 7-d-old seedlings. PP2C-D1 was immunoprecipitated from solubilized microsomal extracts prepared from GFP-SAUR19 or GFP-SAUR19[35S:PP2C-D1] seedlings. Precipitates were then immunoblotted with α-PP2C-D1 (top) and α-GFP (bottom) antibodies. The middle two lanes show control immunoprecipitations using preimmune sera.