Figure 1.

TGBp1 Induces Developmental Defects Resembling Those Observed in tasiRNA-Deficient Mutants and Significantly Reduces tasiRNA Accumulation.

(A) Photographs of 4-week-old TGBp1 transgenic lines (1F, 7A, 10D, and 7D), tasiRNA-deficient mutants (sgs3, rdr6, dcl4, and ago7), TBSV p19, CMV 2b, and GUS transformants, and a wild-type Col-0 plant.

(B) Immunoblot analysis with anti-TGBp1 polyclonal antibody to detect TGBp1 protein levels in ∼5-week-old TGBp1 transgenic lines. Coomassie Brilliant Blue (CBB) staining is shown as a loading control.

(C) RNA gel blot analysis of TAS2 and TAS3 tasiRNAs, miR173, miR390, and miR171. Total RNA was prepared from the indicated transformants or mutants. The numbers below each lane show accumulation levels relative to wild-type Col-0, after normalization against ethidium bromide–stained low-molecular-weight (LMW) RNAs.

(D) Quantitative real-time RT-PCR analysis of PPR and ARF3 mRNA levels in plants at the reproductive stage. Relative expression values (means ± sd, n = 3) were normalized against ACTIN2 mRNA levels.

[See online article for color version of this figure.]