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. 2014 May 13;26(5):2184–2200. doi: 10.1105/tpc.113.120360

Figure 4.

Figure 4.

Root Hair Density and Seed Coat Mucilage Phenotypes Reveal Tissue-Specific Interactions.

(A) to (M) Primary roots from 3- to 4-d-old seedlings.

(A) to (F) Col wild-type (A), hdg11-1 (B), and myb23-3 (E) root hair density appears normal in comparison with excess root hair formation in gl2-5 (C), gl2-5 hdg11-1 (D), and gl2-5 myb23-3 (F).

(G) to (I) Transgenic lines expressing ProGL2:EYFP:hdg11Δ do not appear to alter root hair density in hdg11-1 (G), gl2-5 (H), or gl2-5 hdg11-1 (I).

(J) Expression of ProGL2:EYFP:GL2 rescues the root hair phenotype of gl2-5.

(K) to (M) Expression of ProGL2:EYFP:HDG11 has no effect on root hair density in hdg11-1 seedlings (K). Partial complementation is observed for gl2-5 (L) and possibly gl2-5 hdg11-1 (M) roots (white arrows).

Yellow arrows point to abnormal root hair formation near the tips of the roots.

(A′) to (M′) Seeds were stained with ruthenium red to detect mucilage production upon imbibition.

(A′) to (F′) Col wild-type (A′), hdg11-1 (B′), and myb23-3 (E′) seeds exhibit a normal outer mucilage, while gl2-5 (C′), gl2-5 hdg11-1 (D′), and gl2-5 myb23-3 (F′) lack mucilage.

(G′) to (I′) ProGL2:EYFP:hdg11Δ expression does not affect seed mucilage production in hdg11-1 (G′), gl2-5 (H′), or gl2-5 hdg11-1 (I′).

(J′) Expression of ProGL2:EYFP:GL2 rescues the mucilage phenotype of gl2-5.

(K′) ProGL2:EYFP:HDG11 expression negatively affects mucilage production in hdg11-1 seeds.

(L′) and (M′) gl2-5 (L′) and gl2-5 hdg11-1 (M′) seeds expressing ProGL2:EYFP:HDG11 lack the outer mucilage.

(N) and (O) Quantification of root epidermal cells in genotypes lacking transgenes (N) and expressing EYFP:HDG11 or EYFP:GL2 from transgenes (boxed in red) (O). Percentages of trichoblast cells in root hair cell files (R, brown bars) and atrichoblast cells in non-root-hair cell files (N, gray bars) are indicated (n ≥ 20). Error bars indicate sd. Single asterisks in (N) mark significant differences from the Col wild type, while double asterisks in (O) mark significant differences between mutants with ProGL2:EYFP:hdg11Δ control and ProGL2:EYFP:GL2 or ProGL2:EYFP:HDG11 transgenes expressing wild-type GL2 or HDG11, respectively (two-tiered t test, P < 0.0001).