Figure 3.

Effect of treatment with transforming growth factor β (TGF-β) with or without tumor necrosis factor (TNF)-α on the expression level of Gli family zinc finger 2 (Gli2) in KG-1 cells. KG-1 cells were treated with: no treatment as a control (−), 5 ng/ml transforming growth factor β1 (TGF-β1; +) or 5 ng/ml TGF-β1+5 ng/ml TNF-α (+), respectively for 24 h. (A) The total mRNA in the KG-1 cells was extracted and quantitative PCR was carried out. (B) The total proteins in the KG-1 cells were extracted and western blot analyses were performed. ^*P<0.05, treatment group (+) vs. control (−) group; ^#P<0.05, TGF-β1 + TNF-α group vs. TGF-β1 group.