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. 2014 Jun 3;8(2):557–562. doi: 10.3892/etm.2014.1754

Figure 3.

Figure 3

GTS-21 reduces the percentages of IFN-γ+ T cells in RA CD4+ T cells during Th1 differentiation. (A–C) Gating strategy used for fluorescence-activated cell sorting of CD3+CD8− T cells from patients with RA. (D) Flow cytometric analysis isotype. (E) Percentages of IFN-γ+ cells among CD3+CD8− T cells stimulated by anti-CD3/-CD28 for 72 h. (F) Percentages of IFN-γ+ cells among CD3+CD8− T cells under Th1-differentiation conditions for 72 h. (G and H) Effect of (G) GTS-21 alone or (H) with αBgt on the expression of IFN-γ by CD3+CD8− T cells from patients with RA. Representative results are shown. Numbers are the percentages of cells within the quadrants. (I) Quantification of the percentages of IFN-γ+CD3+CD8− T cells. (J) Expression of IFN-γ in supernatants of differentiated Th1 CD4+ T cell cultures was analyzed by ELISA. Bars show the mean ± standard deviation. *P<0.05. IFN, interferon; RA, rheumatoid arthritis; CD, cluster of differentiation ; αBgt, α-bungarotoxin; Th1, T helper 1; IL, interleukin; SSC, side scatter; FITC, fluorescein isothiocyanate; PE, phycoerythrin.