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. 2014 Jun 2;8(2):527–532. doi: 10.3892/etm.2014.1752

Figure 4.

Figure 4

Overexpression of miR-155 upregulated anticancer drug-induced autophagy in osteosarcoma cells. (A) LC3 punctas under fluorescence microscopy in Saos-2 cells following 0.05 μg/ml Dox or 5 μM Cis treatment and miR-155 mimics or miR-control transfection. (B) Quantitative analysis of the punctate GFP-LC3 dots. (C) Western blotting of LC3-I/II and Atg 5 in miR-155 mimics-transfected Saos-2 cells; (D) Relative expression of LC3-II to LC3-I in miR-155 mimics-transfected Saos-2 cells; (E) Relative expression of Atg 5 to GAPDH in miR-155 mimics-transfected Saos-2 cells. Independent experiments were performed in triplicate. *P<0.05 or **P<0.01, vs. control. miR-155, microRNA-155; Dox, doxorubicin; Cis, cisplatin; GFP, green fluorescent protein; Atg5, autophagy protein 5.