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Modulation of estrogen receptor-α (ERα) and cyclin D1 by genistein in MCF-7 and differentiating 3T3-L1 cells. (A) For determination of ERα, MCF-7 cells were exposed to genistein at 50, 100, 150 and 200 μM for 24, 48 and 72 h. (B) In addition, 3T3-L1 and MCF-7 cells were exposed to 50 μM genistein for 48 h. Values are expressed as means ± standard deviations. CTL, control group (vehicle only); *P<0.05, significantly different from the CTL group.
